Anti-DPF2 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-DPF2 磁珠免疫沉淀(IP)试剂盒产品组分

Components Storage
Anti-DPF2 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-DPF2 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-DPF2 磁珠免疫沉淀(IP)试剂盒产品描述

The Anti-DPF2 magnetic Beads, conjugated with Anti-DPF2 antibody, are used for immuneprecipitation (IP) of DPF2 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing DPF2 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound DPF2 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-DPF2 磁珠免疫沉淀(IP)试剂盒抗体信息

抗体
Anti-DPF2 Antibody, Rabbit Polyclonal(203094-T38)
免疫原
E. coli-derived Human DPF2 fragment
交叉反应
Human DPF2
来源
Polyclonal Human Rabbit IgG
制备方法
Produced in rabbits immunized with E. coli-derived Human DPF2 fragment, and purified by antigen affinity chromatography.
应用
Immunoprecipitation (IP), Minimum Protein Purification

Anti-DPF2 Magnetic Beads Immunoprecipitation (IP) Kit: 别称

Anti-double PHD fingers 2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-REQALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-ubi-d4ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-UBID4ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

DPF2 背景信息

The d4-protein family is characterized by an N-terminal 2/3 domain, a C2H2-type zinc finger (ZF), and a C-terminal tandem PHD zinc finger. The d4 family of transcription factors consists of three members in mammals. DPF1/neuro-d4 is expressed mainly in neurons and the peripheral nervous system, and is important for brain development. DPF2/requiem/ubi-d4 is expressed ubiquitously and presumably functions as an apoptotic factor, especially during the deprivation of trophic factors. Double plant homeodomain finger 2 (DPF2) is a highly evolutionarily conserved member of the d4 protein family that is ubiquitously expressed in human tissues and was recently shown to inhibit the myeloid differentiation of hematopoietic stem/progenitor and acute myelogenous leukemia cells. The histone binding of DPF2 plays an important regulatory role in the transcriptional program that drives myeloid differentiation. DPF3/cer-d4 is expressed in neurons and the heart, and is important for heart development and function in zebrafish. In Drosophila, there is only one member, dd4, whose function is still unknown, but it is expressed in many tissues and is particularly abundant in the brain of developing embryos and in adults.
全称
double PHD fingers 2
参考文献
  • Zhang W, et al. (2011) Crystal structure of the cys2his2-type zinc finger domain of human dpf2. Biochem Biophys Res Commun 413 (1): 58-61.
  • Villanueva-Chimal E, et al. (2017) Dpff-1 transcription factor deficiency causes the aberrant activation of mpk-1 and meiotic defects in the caenorhabditis elegans germline. Genesis 55 (11).
  • Huber FM, et al. (2017) Histone-binding of dpf2 mediates its repressive role in myeloid differentiation. Proc Natl Acad Sci U S A 114 (23): 6016-6021.
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