Anti-EpCAM Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-EpCAM 磁珠免疫沉淀(IP)试剂盒产品组分

Components Storage
Anti-EpCAM Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-EpCAM magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-EpCAM 磁珠免疫沉淀(IP)试剂盒产品描述

The Anti-EpCAM magnetic Beads, conjugated with Anti-EpCAM antibody, are used for immuneprecipitation (IP) of EpCAM proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing EpCAM proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound EpCAM proteins are dissociated from the magnetic beads using an elution buffer.

Anti-EpCAM 磁珠免疫沉淀(IP)试剂盒抗体信息

抗体
Anti-EpCAM Antibody, Rabbit Polyclonal(90299-T60)
免疫原
Recombinant Cynomolgus EpCAM / TROP-1 / TACSTD1 protein (Catalog#90299-CCCH)
交叉反应
Cynomolgus EpCAM / TROP-1 / TACSTD1
来源
Polyclonal Cynomolgus Rabbit IgG
制备方法
Produced in rabbits immunized with purified, recombinant Cynomolgus EpCAM / TROP-1 / TACSTD1 (Catalog#90299-CCCH; EHH55554.1; Met1-Lys265). EpCAM / TROP-1 / TACSTD1 specific IgG was purified by Cynomolgus EpCAM / TROP-1 / TACSTD1 affinity chromatography.
应用
Immunoprecipitation (IP), Minimum Protein Purification

EpCAM 背景信息

Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type â…transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway.
全称
epithelial cell adhesion molecule
参考文献
  • Brunner A, et al. (2008) EpCAM is predominantly expressed in high grade and advanced stage urothelial carcinoma of the bladder. J Clin Pathol. 61(3):307-10.
  • Trzpis M, et al. (2008) EpCAM in morphogenesis. Front Biosci. 13: 5050-5.
  • Munz M, et al. (2009) The emerging role of EpCAM in cancer and stem cell signaling. Cancer Res. 69(14): 5627-9.
  • Carpenter G, et al. (2009) EpCAM: another surface-to-nucleus missile. Cancer Cell. 15(3): 165-6.
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