Anti-IFT20 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-IFT20 磁珠免疫沉淀(IP)试剂盒产品组分

Components Storage
Anti-IFT20 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-IFT20 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-IFT20 磁珠免疫沉淀(IP)试剂盒产品描述

The Anti-IFT20 magnetic Beads, conjugated with Anti-IFT20 antibody, are used for immuneprecipitation (IP) of IFT20 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing IFT20 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound IFT20 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-IFT20 磁珠免疫沉淀(IP)试剂盒抗体信息

抗体
Anti-IFT20 Antibody, Rabbit Polyclonal(203666-T44)
免疫原
E. coli-derived Human IFT20/intraflagellar transport 20 fragment
交叉反应
Human IFT20/intraflagellar transport 20
来源
Polyclonal Human Rabbit IgG
制备方法
Produced in rabbits immunized with E. coli-derived Human IFT20/intraflagellar transport 20 fragment, and purified by antigen affinity chromatography.
应用
Immunoprecipitation (IP), Minimum Protein Purification

IFT20 背景信息

Eukaryotic cilia are assembled via intraflagellar transport (IFT) in which large protein particles motor along ciliary microtubules. IFT20 functions in the delivery of ciliary membrane proteins from the Golgi complex to the cilium. IFT20 is the smallest member of the intraflagellar transport protein (IFT) complex B. It is involved in cilia formation. IFT20 in the early stage of T-cell development inhibited collagen-induced arthritis (CIA) development through regulating T-cell development and the expression of critical cytokines. The intraflagellar transport protein 20 (IFT20), a component of the intraflagellar transport system, controls polarized traffic during immune synapse assembly. IFT20 is therefore required for the delivery of the intracellular pool of LAT to the immune synapse in naive primary T lymphocytes and for effective T-cell responses in vivo. IFT20 is essential for male fertility and spermiogenesis in mice, and its major function is to transport cargo proteins for sperm flagella formation.
全称
intraflagellar transport 20
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