Anti-MAP7 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-MAP7 磁珠免疫沉淀(IP)试剂盒产品组分

Components Storage
Anti-MAP7 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-MAP7 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-MAP7 磁珠免疫沉淀(IP)试剂盒产品描述

The Anti-MAP7 magnetic Beads, conjugated with Anti-MAP7 antibody, are used for immuneprecipitation (IP) of MAP7 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing MAP7 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound MAP7 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-MAP7 磁珠免疫沉淀(IP)试剂盒抗体信息

抗体
Anti-MAP7 Antibody, Rabbit Polyclonal(204051-T46)
免疫原
E. coli-derived Human MAP7 fragment
交叉反应
Human MAP7
来源
Polyclonal Human Rabbit IgG
制备方法
Produced in rabbits immunized with E. coli-derived Human MAP7 fragment, and purified by antigen affinity chromatography.
应用
Immunoprecipitation (IP), Minimum Protein Purification

Anti-MAP7 Magnetic Beads Immunoprecipitation (IP) Kit: 别称

Anti-E-MAP-115ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-EMAP115ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

MAP7 背景信息

MAP7 (ensconsin, E-MAP-115) is a ubiquitous MAP that organizes the microtubule cytoskeleton in mitosis and neuronal branching. Microtubule-associated protein MAP7 (ensconsin in Drosophila) is an essential kinesin-1 cofactor and promotes kinesin-1 recruitment to microtubules. MAP7 is a unique microtubule-associated protein that interacts with both microtubules and the motor protein kinesin-1. Structure-function analysis in rat embryonic sensory neurons shows that the kinesin-1 interacting domain in MAP7 is required for axon and branch growth but not for branch formation. Also, two unique microtubule-binding sites are found in MAP7 that have distinct dissociation kinetics and are both required for branch formation. Furthermore, MAP7 recruits kinesin-1 dynamically to microtubules, leading to alterations in organelle transport behaviors, particularly pause/speed switching. Microtubule-associated protein 7 (MAP7) plays an important role in cancer cells.
全称
microtubule-associated protein 7
参考文献
  • Tymanskyj SR, et al. (2018) Map7 regulates axon morphogenesis by recruiting kinesin-1 to microtubules and modulating organelle transport. Elife 7: e36374.
  • Pan X, et al. (2019) Map7d2 localizes to the proximal axon and locally promotes kinesin-1-mediated cargo transport into the axon. Cell Rep 26 (8): 1988-1999.e1986.
  • Chaudhary AR, et al. (2019) Map7 regulates organelle transport by recruiting kinesin-1 to microtubules. J Biol Chem 294 (26): 10160-10171.
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