Anti-MLPH/Melanophilin Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-MLPH/Melanophilin 磁珠免疫沉淀(IP)试剂盒产品组分

Components Storage
Anti-MLPH/Melanophilin Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-MLPH/Melanophilin magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-MLPH/Melanophilin 磁珠免疫沉淀(IP)试剂盒产品描述

The Anti-MLPH/Melanophilin magnetic Beads, conjugated with Anti-MLPH/Melanophilin antibody, are used for immuneprecipitation (IP) of MLPH/Melanophilin proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing MLPH/Melanophilin proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound MLPH/Melanophilin proteins are dissociated from the magnetic beads using an elution buffer.

Anti-MLPH/Melanophilin 磁珠免疫沉淀(IP)试剂盒抗体信息

抗体
Anti-MLPH/Melanophilin Antibody, Rabbit Polyclonal(203670-T44)
免疫原
E. coli-derived Human MLPH/Melanophilin fragment
交叉反应
Human MLPH/Melanophilin
来源
Polyclonal Human Rabbit IgG
制备方法
Produced in rabbits immunized with E. coli-derived Human MLPH/Melanophilin fragment, and purified by antigen affinity chromatography.
应用
Immunoprecipitation (IP), Minimum Protein Purification

Anti-MLPH/Melanophilin Magnetic Beads Immunoprecipitation (IP) Kit: 别称

Anti-SLAC2-AALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

MLPH/Melanophilin 背景信息

Melanophilin (Mlph), a protein complex involving myosin Va (MyoVa) and Rab27a. Melanophilin (Mlph) forms an interaction with Rab27a and the actin-based motor protein MyosinVa (MyoVa) on mature melanosome membranes and the tripartite complex regulates melanosome transport in melanocytes. Mlph regulates the retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Mlph is proposed to be a modular protein-binding the melanosome-associated protein Rab27a, Myosin Va (MyoVa), actin, and microtubule end-binding protein (EB1), via distinct N-terminal Rab27a-binding domain (R27BD), medial MyoVa-binding domain (MBD), and C-terminal actin-binding domain (ABD), respectively. Loss-of-function variants in the melanophilin gene (MLPH) cause a recessively inherited form of coat color dilution in many mammalian and avian species including the dog.
全称
melanophilin
参考文献
  • Park JI, et al. (2019) The absence of rab27a accelerates the degradation of melanophilin. Exp Dermatol 28 (1): 90-93.
  • Lee JA, et al. (2019) Identification of microrna targeting mlph and affecting melanosome transport. Biomolecules 9 (7): 265.
  • Hume AN, et al. (2006) A coiled-coil domain of melanophilin is essential for myosin va recruitment and melanosome transport in melanocytes. Mol Biol Cell 17 (11): 4720-4735.
  • Bauer A, et al. (2018) A novel mlph variant in dogs with coat colour dilution. Anim Genet 49 (1): 94-97.
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