Anti-MRPL20 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-MRPL20 磁珠免疫沉淀(IP)试剂盒产品组分

Components Storage
Anti-MRPL20 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-MRPL20 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-MRPL20 磁珠免疫沉淀(IP)试剂盒产品描述

The Anti-MRPL20 magnetic Beads, conjugated with Anti-MRPL20 antibody, are used for immuneprecipitation (IP) of MRPL20 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing MRPL20 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound MRPL20 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-MRPL20 磁珠免疫沉淀(IP)试剂盒抗体信息

抗体
Anti-MRPL20 Antibody, Rabbit Polyclonal(203671-T44)
免疫原
E. coli-derived Human MRPL20 fragment
交叉反应
Human MRPL20
来源
Polyclonal Human Rabbit IgG
制备方法
Produced in rabbits immunized with E. coli-derived Human MRPL20 fragment, and purified by antigen affinity chromatography.
应用
Immunoprecipitation (IP), Minimum Protein Purification

Anti-MRPL20 Magnetic Beads Immunoprecipitation (IP) Kit: 别称

Anti-L20mtALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-MRP-L20ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

MRPL20 背景信息

MRPL20 (Mitochondrial Ribosomal Protein L20) is a Protein Coding gene. The MRPL20 gene, located on 1p36.33, is conserved in Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, A.thaliana, rice, and frog. MRPL20 belongs to the bacterial ribosomal protein bL20 family. This gene encodes a 39S subunit protein and is widely expressed in kidney, fat, and other tissues. A pseudogene corresponding to this gene is found on chromosome 21q. Alternative splicing results in multiple transcript variants encoding different isoforms. Among its related pathways are Organelle biogenesis and maintenance and Viral mRNA Translation. 241 organisms have orthologs with the human gene MRPL20.
全称
mitochondrial ribosomal protein L20
参考文献
  • Koc EC, et al. (2001) The large subunit of the mammalian mitochondrial ribosome. Analysis of the complement of ribosomal proteins present. J Biol Chem 276 (47): 43958-43969.
  • Haque ME, et al. (2010) Properties of the c-terminal tail of human mitochondrial inner membrane protein oxa1l and its interactions with mammalian mitochondrial ribosomes. J Biol Chem 285 (36): 28353-28362.
  • Arroyo JD, et al. (2016) A genome-wide crispr death screen identifies genes essential for oxidative phosphorylation. Cell Metab 24 (6): 875-885.
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