Anti-NAA10/ARD1 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-NAA10/ARD1 磁珠免疫沉淀(IP)试剂盒产品组分

Components Storage
Anti-NAA10/ARD1 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-NAA10/ARD1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-NAA10/ARD1 磁珠免疫沉淀(IP)试剂盒产品描述

The Anti-NAA10/ARD1 magnetic Beads, conjugated with Anti-NAA10/ARD1 antibody, are used for immuneprecipitation (IP) of NAA10/ARD1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing NAA10/ARD1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound NAA10/ARD1 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-NAA10/ARD1 磁珠免疫沉淀(IP)试剂盒抗体信息

抗体
Anti-NAA10/ARD1 Antibody, Rabbit Polyclonal(101566-T46)
免疫原
A synthetic peptide corresponding to the N-terminus of the Human ARD1A/NAA10
交叉反应
Human Mouse (Species predicted to react based on 100% sequence homology)
来源
Polyclonal Human Rabbit IgG
制备方法
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human ARD1A/NAA10, and purified by antigen affinity chromatography.
应用
Immunoprecipitation (IP), Minimum Protein Purification

Anti-NAA10/ARD1 Magnetic Beads Immunoprecipitation (IP) Kit: 别称

Anti-ARD1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-ARD1AALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-ARD1PALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-DXS707ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-MCOPS1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-NAA10ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-NATDALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-OGDNSALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-TE2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

NAA10/ARD1 背景信息

ARD1 is a member of the 2-kDa ARF protein family. It is a multifunctional protein. ARD1 has an 18-kDa ADP-ribosylation factor (ARF) domain at the C-terminus (amino acids 43-574), and a 46-kDa N-terminal domain (amino acids 1-42). The C-terminal region of ARD1 may be involved in the formation of both ARD1-ARD1 and ARD1-NAT1 complexes. ARD1 and NAT1 genes are required for the expression of an N-terminal protein acetyltransferase. This activity is required for full repression of the silent mating-type locus HML, for sporulation, and for entry into G. Recombinant ARD1 (amino acids 1-574) or its RING finger domain (amino acids 1-11) produced polyubiquitylated proteins when incubated in vitro with a mammalian E1, an E2 enzyme, ATP, and ubiquitin.
全称
N(alpha)-acetyltransferase 10, NatA catalytic subunit
参考文献
  • Tribioli C., et al.,(1994), Isolation of new genes in distal Xq28: transcriptional map and identification of a human homologue of the ARD1 N-acetyl transferase of Saccharomyces cerevisiae. Hum. Mol. Genet. 3:1061-1068.
  • Arnesen T., et al., (2005), Identification and characterization of the human ARD1-NATH protein acetyltransferase complex.Biochem. J. 386:433-443.
  • Ross M.T., et al.,(2005), The DNA sequence of the human X chromosome.Nature 434:325-337.
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