Anti-PCYT1A Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-PCYT1A 磁珠免疫沉淀(IP)试剂盒产品组分

Components Storage
Anti-PCYT1A Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-PCYT1A magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-PCYT1A 磁珠免疫沉淀(IP)试剂盒产品描述

The Anti-PCYT1A magnetic Beads, conjugated with Anti-PCYT1A antibody, are used for immuneprecipitation (IP) of PCYT1A proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing PCYT1A proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound PCYT1A proteins are dissociated from the magnetic beads using an elution buffer.

Anti-PCYT1A 磁珠免疫沉淀(IP)试剂盒抗体信息

抗体
Anti-PCYT1A Antibody, Rabbit Polyclonal(202950-T44)
免疫原
E. coli-derived Human PCYT1A fragment
交叉反应
Human PCYT1A
来源
Polyclonal Human Rabbit IgG
制备方法
Produced in rabbits immunized with E. coli-derived Human PCYT1A fragment, and purified by antigen affinity chromatography.
应用
Immunoprecipitation (IP), Minimum Protein Purification

Anti-PCYT1A Magnetic Beads Immunoprecipitation (IP) Kit: 别称

Anti-CCTAALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-CTALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-CTAALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-CTPCTALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-PCYT1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-SMDCRDALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

PCYT1A 背景信息

PCYT1A, the rate-limiting enzyme of Phosphatidylcholine (PC) synthesis, is intranuclear and re-locates to the nuclear membrane in response to the need for membrane PL synthesis in yeast, fly, and mammalian cells. PCYT1A is associated with cancer progression. PCYT1A suppresses proliferation and migration by inhibiting the mTORC1 signaling pathway in lung adenocarcinoma. PCYT1A shows as a potential promising biomarker in lung adenocarcinoma. Mutations in the PCYT1A gene have been recently linked to two different phenotypes: one characterized by spondylometaphyseal dysplasia and cone-rod dystrophy (SMD-CRD) and the other by congenital lipodystrophy, severe fatty liver disease, and reduced HDL cholesterol without any retinal or skeletal involvement.
全称
phosphate cytidylyltransferase 1, choline, alpha isoform
参考文献
  • Yu J, et al. (2020) Pcyt1a suppresses proliferation and migration via inhibiting mtorc1 pathway in lung adenocarcinoma. Biochem Biophys Res Commun 529 (2): 353-361.
  • Testa F, et al. (2017) Mutations in the pcyt1a gene are responsible for isolated forms of retinal dystrophy. Eur J Hum Genet 25 (5): 651-655.
  • Haider A, et al. (2018) Pcyt1a regulates phosphatidylcholine homeostasis from the inner nuclear membrane in response to membrane stored curvature elastic stress. Dev Cell 45 (4): 481-495.e488.
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