Anti-PGAM1 Magnetic Beads Immunoprecipitation (IP) Kit

Temporarily not available outside of China.
Price:
Size:
Number:

Anti-PGAM1 磁珠免疫沉淀(IP)试剂盒产品组分

Components Storage
Anti-PGAM1 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-PGAM1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-PGAM1 磁珠免疫沉淀(IP)试剂盒产品描述

The Anti-PGAM1 magnetic Beads, conjugated with Anti-PGAM1 antibody, are used for immuneprecipitation (IP) of PGAM1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing PGAM1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound PGAM1 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-PGAM1 磁珠免疫沉淀(IP)试剂盒抗体信息

抗体
Anti-PGAM1 Antibody, Rabbit Polyclonal(202955-T46)
免疫原
E. coli-derived Human PGAM1 fragment
交叉反应
Human PGAM1
来源
Polyclonal Human Rabbit IgG
制备方法
Produced in rabbits immunized with E. coli-derived Human PGAM1 fragment, and purified by antigen affinity chromatography.
应用
Immunoprecipitation (IP), Minimum Protein Purification

Anti-PGAM1 Magnetic Beads Immunoprecipitation (IP) Kit: 别称

Anti-HEL-S-35ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-PGAM-BALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-PGAMAALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

PGAM1 背景信息

Phosphoglycerate mutase 1 (PGAM1) is a glycolytic enzyme that coordinates glycolysis and biosynthesis to promote cancer growth via its metabolic activity. PGAM1 plays a pivotal role in cancer metabolism and tumor progression via its metabolic activity and interaction with other proteins like α-smooth muscle actin (ACTA2). Allosteric regulation is considered to be an innovative strategy to discover a highly selective and potent inhibitor targeting PGAM1. PGAM1 is upregulated in many cancer types and involved in cell proliferation, migration, invasion, and apoptosis. PGAM1 may play an important role in prostate cancer progression and aggressiveness, and that it might be a valuable marker of poor prognosis and a potential therapeutic target for prostate cancer.
全称
phosphoglycerate mutase 1 (brain)
参考文献
  • Zhang D, et al. (2020) Correction: Phosphoglycerate mutase 1 promotes cancer cell migration independent of its metabolic activity. Oncogene 39 (11): 2451-2452.
  • Wen YA, et al. (2018) Phosphoglycerate mutase 1 knockdown inhibits prostate cancer cell growth, migration, and invasion. Asian J Androl 20 (2): 178-183.
  • Wen CL, et al. (2019) An allosteric pgam1 inhibitor effectively suppresses pancreatic ductal adenocarcinoma. Proc Natl Acad Sci U S A 116 (46): 23264-23273.
  • Huang K, et al. (2019) A novel allosteric inhibitor of phosphoglycerate mutase 1 suppresses growth and metastasis of non-small-cell lung cancer. Cell Metab 30 (6): 1107-1119.e1108.
添加购物车成功! 添加购物车失败!请再次尝试 正在更新购物车,请稍后 U.S.A.