Anti-PLBL2 / PLBD2 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-PLBL2 / PLBD2 磁珠免疫沉淀(IP)试剂盒产品组分

Components Storage
Anti-PLBL2 / PLBD2 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-PLBL2 / PLBD2 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-PLBL2 / PLBD2 磁珠免疫沉淀(IP)试剂盒产品描述

The Anti-PLBL2 / PLBD2 magnetic Beads, conjugated with Anti-PLBL2 / PLBD2 antibody, are used for immuneprecipitation (IP) of PLBL2 / PLBD2 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing PLBL2 / PLBD2 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound PLBL2 / PLBD2 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-PLBL2 / PLBD2 磁珠免疫沉淀(IP)试剂盒抗体信息

抗体
Anti-PLBL2 / PLBD2 Antibody, Rabbit Polyclonal(13947-T60)
免疫原
Recombinant Human PLBD2 Protein (Catalog#13947-H08H)
交叉反应
Human PLBD2
来源
Polyclonal Human Rabbit IgG
制备方法
Produced in rabbits immunized with purified, recombinant Human PLBD2 (rh PLBD2; Catalog#13947-H08H; Q8NHP8; Met1-Asp589). PLBD2 specific IgG was purified by Human PLBD2 affinity chromatography.
应用
Immunoprecipitation (IP), Minimum Protein Purification

Anti-PLBL2 / PLBD2 Magnetic Beads Immunoprecipitation (IP) Kit: 别称

Anti-P76ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

PLBL2 / PLBD2 背景信息

PLBD2 localizes to the lysosome, as its absence could plausibly lead to a serious yet unrecognized lysosomal storage disease. PLBD1 and PLBD2 are semi-orphans in the sense of being probable phospholipases of B class but with uncertain physiological substrates and thus functionalities. PLBD1 and PLBD2 constitute a small gene family (sequence homology class) within vertebrates though one that occurs expanded in some early diverging eukaryotes. PLBD2 presents a special difficulty in that a sequence of post-translational steps are apparently necessary for its activation. Without these, potential substrates can hardly be assayed. These steps include removal of the signal peptide, mannosylation appropriate to the lysosome targeting receptor, and self-catalytic proteolytic activation to expose the substrate binding site as this becomes appropriate.
全称
phospholipase B domain containing 2
参考文献
  • Morgan CP. et al., 2004), Biochem J. 382 (2): 441-9.
  • Kim W. et al., 2011, Mol Cell. 44 (2): 325-40.
  • Havugimana PC. et al., 2012. Cell. 150 (5): 1068-81.
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