Anti-PR-Set7 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-PR-Set7 磁珠免疫沉淀(IP)试剂盒产品组分

Components Storage
Anti-PR-Set7 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-PR-Set7 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-PR-Set7 磁珠免疫沉淀(IP)试剂盒产品描述

The Anti-PR-Set7 magnetic Beads, conjugated with Anti-PR-Set7 antibody, are used for immuneprecipitation (IP) of PR-Set7 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing PR-Set7 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound PR-Set7 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-PR-Set7 磁珠免疫沉淀(IP)试剂盒抗体信息

抗体
Anti-PR-Set7 Antibody, Rabbit Polyclonal(11726-T52)
免疫原
Recombinant Human PR-Set7/KMT5A Protein (Catalog#11726-HNCE)
交叉反应
Human PR-Set7/KMT5A
来源
Polyclonal Human Rabbit IgG
制备方法
Produced in rabbits immunized with purified, recombinant Human PR-Set7/KMT5A (rh PR-Set7/KMT5A; Catalog#11726-HNCE; NP_065115.3; Lys195-His352). PR-Set7/KMT5A specific IgG was purified by Human PR-Set7/KMT5A affinity chromatography.
应用
Immunoprecipitation (IP), Minimum Protein Purification

Anti-PR-Set7 Magnetic Beads Immunoprecipitation (IP) Kit: 别称

Anti-KMT5AALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-PR-Set7ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-SET07ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-SET8ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

PR-Set7 背景信息

KMT5A (known as PR-Set7/9, SETD8 and SET8), a member of the SET domain containing methyltransferase family specifically targeting H4K20 for methylation, has been implicated in multiple biological processes. Inhibition of KMT5A attenuated proliferation and induced apoptosis. Elevated KMT5A expression was significantly correlated with extrathyroidal extension, lymph node metastasis and advanced pathological stage of papillary thyroid cancer. KMT5A may be a novel oncogenic factor, specifically a regulator for lipid metabolism in papillary thyroid carcinoma.
全称
SET domain containing (lysine methyltransferase) 8
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