Anti-RBM14 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-RBM14 磁珠免疫沉淀(IP)试剂盒产品组分

Components Storage
Anti-RBM14 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-RBM14 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-RBM14 磁珠免疫沉淀(IP)试剂盒产品描述

The Anti-RBM14 magnetic Beads, conjugated with Anti-RBM14 antibody, are used for immuneprecipitation (IP) of RBM14 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing RBM14 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound RBM14 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-RBM14 磁珠免疫沉淀(IP)试剂盒抗体信息

抗体
Anti-RBM14 Antibody, Rabbit Polyclonal(203140-T46)
免疫原
E. coli-derived Human RBM14 fragment
交叉反应
Human RBM14
来源
Polyclonal Human Rabbit IgG
制备方法
Produced in rabbits immunized with E. coli-derived Human RBM14 fragment, and purified by antigen affinity chromatography.
应用
Immunoprecipitation (IP), Minimum Protein Purification

Anti-RBM14 Magnetic Beads Immunoprecipitation (IP) Kit: 别称

Anti-COAAALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-PSP2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-SIPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-SYTIP1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-TMEM137ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

RBM14 背景信息

RBM14 is a component of the para-speckle complex, which has been implicated in multiple important biological processes. RBM14, is known to function in transcription and RNA splicing. RBM14 is also required for maintaining the stem-like state of GBM spheres, and it controls the DNA-PK-dependent non-homologous end-joining (NHEJ) pathway by interacting with KU80. RBM14 is a RNA-binding protein (RBP) with low complexity domains, called intrinsically disordered proteins (IDPs), and it also physically interacts with PARP1. RBM14 is recruited to DNA double-strand breaks (DSBs) in a poly(ADP-ribose) (PAR)-dependent manner. DNA-dependent PARP1 (poly-(ADP) ribose polymerase 1) makes key contributions in the DNA damage response (DDR) network. RBM14 plays an important role in a PARP-dependent DSB repair process. RBM14 not only plays an important role in maintaining the pluripotency of mouse embryonic stem cells (mESCs) but is also indispensable for mesoderm development.
参考文献
  • Yuan M, et al. (2014) Rna binding protein rbm14 promotes radio-resistance in glioblastoma by regulating DNA repair and cell differentiation. Oncotarget 5 (9): 2820-2826.
  • Kai M (2016) Roles of rna-binding proteins in DNA damage response. Int J Mol Sci 17 (3): 310.
  • Budhiraja S, et al. (2015) Mining the human complexome database identifies rbm14 as an xpo1-associated protein involved in hiv-1 rev function. J Virol 89 (7): 3557-3567.
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