Anti-TGF beta 1 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-TGF beta 1 磁珠免疫沉淀(IP)试剂盒产品组分

Components Storage
Anti-TGF beta 1 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-TGF beta 1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-TGF beta 1 磁珠免疫沉淀(IP)试剂盒产品描述

The Anti-TGF beta 1 magnetic Beads, conjugated with Anti-TGF beta 1 antibody, are used for immuneprecipitation (IP) of TGF beta 1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing TGF beta 1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound TGF beta 1 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-TGF beta 1 磁珠免疫沉淀(IP)试剂盒抗体信息

抗体
Anti-TGF beta 1 Antibody, Rabbit Polyclonal(70087-T52)
免疫原
Recombinant Canine TGF-beta 1 / TGFB1 protein (Catalog#70087-D08H)
交叉反应
Canine TGF-beta 1 / TGFB1
来源
Polyclonal Canine Rabbit IgG
制备方法
Produced in rabbits immunized with purified, recombinant Canine TGF-beta 1 / TGFB1 (Catalog#70087-D08H; ; Met1-Ser390). TGF-beta 1 / TGFB1 specific IgG was purified by Canine TGF-beta 1 / TGFB1 affinity chromatography.
应用
Immunoprecipitation (IP), Minimum Protein Purification

TGF beta 1 背景信息

TGF-beta 1 is a member of the transforming growth factor beta (TGF-beta) family. The transforming growth factor-beta family of polypeptides are involved in the regulation of cellular processes, including cell division, differentiation, motility, adhesion and death. TGF-beta 1 positively and negatively regulates many other growth factors. It inhibits the secretion and activity of many other cytokines including interferon-γ, tumor necrosis factor-alpha and various interleukins. It can also decrease the expression levels of cytokine receptors. Meanwhile, TGF-beta 1 also increases the expression of certain cytokines in T cells and promotes their proliferation, particularly if the cells are immature. TGF-beta 1 also inhibits proliferation and stimulates apoptosis of B cells, and plays a role in controlling the expression of antibody, transferrin and MHC class II proteins on immature and mature B cells. As for myeloid cells, TGF-beta 1can inhibit their proliferation and prevent their production of reactive oxygen and nitrogen intermediates. However, as with other cell types, TGF-beta 1 also has the opposite effect on cells of myeloid origin. TGF-beta 1 is a multifunctional protein that controls proliferation, differentiation and other functions in many cell types. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Once cells lose their sensitivity to TGF-beta1-mediated growth inhibition, autocrine TGF-beta signaling can promote tumorigenesis. Elevated levels of TGF-beta1 are often observed in advanced carcinomas, and have been correlated with increased tumor invasiveness and disease progression.
全称
transforming growth factor, beta 1
Related Pathways
  • P38 MAPK Signaling Pathway
    P38 MAPK Signaling Pathway
参考文献
  • Ghadami M, et al. (2000) Genetic Mapping of the Camurati-Engelmann Disease Locus to Chromosome 19q13.1-q13.3. Am J Hum. Genet. 66(1):143-7.
  • Letterio J, et al. (1998) Regulation of immune responses by TGF-beta. Annu Rev Immunol. 16:137-61.
  • Vaughn SP, et al. (2000) Confirmation of the mapping of the Camurati-Englemann locus to 19q13. 2 and refinement to a 3.2-cM region. Genomics. 66(1):119-21.
  • Assoian R, et al. (1983) Transforming growth factor-beta in human platelets. Identification of a major storage site, purification, and characterization. J Biol Chem. 258(11):7155-60.
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