Anti-TST Magnetic Beads Immunoprecipitation (IP) Kit

Temporarily not available outside of China.

Anti-TST 磁珠免疫沉淀(IP)试剂盒产品组分

Components Storage
Anti-TST Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-TST magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-TST 磁珠免疫沉淀(IP)试剂盒产品描述

The Anti-TST magnetic Beads, conjugated with Anti-TST antibody, are used for immuneprecipitation (IP) of TST proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing TST proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound TST proteins are dissociated from the magnetic beads using an elution buffer.

Anti-TST 磁珠免疫沉淀(IP)试剂盒抗体信息

Anti-TST Antibody, Rabbit Polyclonal(203497-T46)
E. coli-derived Human TST fragment
Human TST
Polyclonal Human Rabbit IgG
Produced in rabbits immunized with E. coli-derived Human TST fragment, and purified by antigen affinity chromatography.
Immunoprecipitation (IP), Minimum Protein Purification

Anti-TST Magnetic Beads Immunoprecipitation (IP) Kit: 别称

Anti-LL22NC01-146D10.3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-RDSALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

TST 背景信息

Thiosulfate sulfurtransferase (TST, EC, also known as Rhodanese, is a mitochondrial enzyme that catalyzes the transfer of sulfur in several molecular pathways. The TST gene, located on 22q12.3, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, C.elegans, and frog. This is one of two neighboring genes encoding similar proteins that each contain two rhodanese domains. The encoded protein is localized to the mitochondria and catalyzes the conversion of thiosulfate and cyanide to thiocyanate and sulfite. Also, the protein interacts with 5S ribosomal RNA and facilitates its import into the mitochondria. TST is widely expressed in the colon, liver, and other tissues. TST deficiency was shown to be strongly related to the pathophysiology of metabolic diseases including diabetes and obesity.
thiosulfate sulfurtransferase (rhodanese)
  • Włodek P, et al. (2010) Thiol levels, protein carbonylation and anaerobic sulfur metabolism in erythrocytes of peritoneal dialysis and predialysis patients. Nephrology (Carlton) 15 (8): 755-761.
  • Libiad M, et al. (2015) Polymorphic variants of human rhodanese exhibit differences in thermal stability and sulfur transfer kinetics. J Biol Chem 290 (39): 23579-23588.
  • Kruithof PD, et al. (2020) Unraveling the role of thiosulfate sulfurtransferase in metabolic diseases. Biochim Biophys Acta Mol Basis Dis 1866 (6): 165716.
添加购物车成功! 添加购物车失败!请再次尝试 正在更新购物车,请稍后 U.S.A.