Macaca fascicularis Cytomegalovirus (CyCMV) (strain OT-1) glycoprotein B / GB HEK293 Cell Lysate (WB positive control)

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Macaca fascicularis Cytomegalovirus (CyCMV) (strain OT-1) glycoprotein B / GB HEK293 Cell Lysate (WB positive control): 产品信息

产品描述
This CyCMV CyCMV Glycoprotein B overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CyCMV Glycoprotein B protein (Cat: 40369-V02H) from the overexpression lysate was verified.
表达宿主
HEK293 Cells
种属
CyCMV
蛋白构建信息
A DNA sequence encoding the macaca fascicularis cytomegalovirus (CyCMV)(strain OT-1) glycoprotein B(gB) (ADV35145.1) (Met1-Asp680, with cleavage site mutated ‘RHKR’ to ‘TTQT’) was expressed with the Fc region of human IgG1 at the C-terminus.
分子量
The recombinant macaca fascicularis cytomegalovirus (CyCMV)(strain OT-1) glycoprotein B(gB) consists 899 amino acids and predicts a molecular mass of 102.5 kDa.

Macaca fascicularis Cytomegalovirus (CyCMV) (strain OT-1) glycoprotein B / GB HEK293 Cell Lysate (WB positive control): 使用指南

制备方法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解缓冲液
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
使用建议
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
缓冲液
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
应用
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Macaca fascicularis Cytomegalovirus (CyCMV) (strain OT-1) glycoprotein B / GB HEK293 Cell Lysate (WB positive control): 别称

CyCMV GB Overexpression Lysate
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