Human Allergin 1 HEK293 Overexpression Lysate


Human Allergin 1 HEK293 Overexpression Lysate: 产品信息

This Human Allergin 1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Allergin 1 protein (Cat: 16083-H02H) from the overexpression lysate was verified.
HEK293 Cells
A DNA sequence encoding the human MILR1 (NP_001078892.1) (Met1-Lys227) was expressed with the Fc region of human IgG1 at the C-terminus.
The recombinant human MILR1 consists of 446 amino acids and predicts a molecular mass of 50.1 kDa.

Human Allergin 1 HEK293 Overexpression Lysate: 使用指南

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human Allergin 1 HEK293 Overexpression Lysate: 别称

Human Allergin-1 Overexpression Lysate; Human C17orf60 Overexpression Lysate; Human MCA-32 Overexpression Lysate; Human MCA32 Overexpression Lysate

Allergin 1 背景信息

MILR1 (Mast Cell Immunoglobulin Like Receptor 1, also known as MCA32) is a Protein Coding gene. It is broadly expressed in the appendix, lymph node, and other tissues. Cell surface immunoreceptor MILR1 has been shown to suppress immunoglobulin E (IgE)-mediated, mast cell-dependent responses in both mice and humans. The C allele of rs6504230 was associated with increased expression of MILR1, which was following the results of expression quantitative trait loci analysis using human leukocytes. The rs6504230 polymorphism affects MILR1 expression levels in humans, leading to a susceptibility to producing specific IgE antibodies against common allergens. Diseases associated with MILR1 include Mitochondrial Dna Depletion Syndrome 16 and Autosomal Dominant Progressive External Ophthalmoplegia.
mast cell immunoglobulin-like receptor 1
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