Human Zinc Alpha 2 Glycoprotein/AZGP1 HEK293 Overexpression Lysate: 产品信息
This Human Zinc Alpha 2 Glycoprotein/AZGP1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Zinc Alpha 2 Glycoprotein/AZGP1 protein (Cat: 13242-H08H) from the overexpression lysate was verified.
A DNA sequence encoding the human AZGP1 (P25311) (Met1-Ser298) with a C-terminal polyhistidine tag was expressed.
The recombinant human AZGP1 comprises 289 amino acids and has a predicted molecular mass of 33.6 kDa. The apparent molecular mass of the protein is approximately 41 kDa in SDS-PAGE under reducing conditions.
Human Zinc Alpha 2 Glycoprotein/AZGP1 HEK293 Overexpression Lysate: 使用指南
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human Zinc Alpha 2 Glycoprotein/AZGP1 HEK293 Overexpression Lysate: 别称
Human ZA2G Overexpression Lysate; Human ZAG Overexpression Lysate
Zinc Alpha 2 Glycoprotein/AZGP1 背景信息
Alpha-2-glycoprotein, also known as AZGP1, belongs to the MHC class I family. It can be detected in body fluids such as serum, sweat, and seminal and breast cyst fluids. It has been shown that alpha-2-glycoprotein can stimulate lipolysis by adipocytes in vivo and in vitro. Thus it is believed that alpha-2-glycoprotein plays an important role in the regulation of body weight, and age-dependent changes in genetically influenced obesity, and it also regulates melanin production by normal and malignant melanocytes. Alpha-2-glycoprotein is produced by both white and brown fat adipocytes and may act in a local autocrine fashion in the reduction of adiposity in cachexia.
alpha-2-glycoprotein 1, zinc-binding
Tada T, et al. (1991) Immunohistochemical localization of Zn-alpha 2-glycoprotein in normal human tissues. J Histochem Cytochem. 39(9):1221-6.
Vanni H, et al. (2009) Cigarette Smoking Induces Overexpression of a Fat-Depleting Gene AZGP1 in the Human. Chest. 135(5):1197-208.
Ueyama H, et al. (1991) Cloning and nucleotide sequence of a human Zn-alpha 2-glycoprotein cDNA and chromosomal assignment of its gene. Biochem Biophys Res Commun. 177(2):696-703.