Human beta Amylase/AMY2B HEK293 Overexpression Lysate: 产品信息
This Human beta Amylase/AMY2B overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of beta Amylase/AMY2B protein (Cat: 13575-H02H) from the overexpression lysate was verified.
A DNA sequence encoding the human AMY2B (NP_066188.1) (Met1-Leu511) was expressed with the Fc region of human IgG1 at the C-terminus.
The recombinant human AMY2B consists 732 amino acids and predicts a molecular mass of 82.4 kDa.
Human beta Amylase/AMY2B HEK293 Overexpression Lysate: 使用指南
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human beta Amylase/AMY2B HEK293 Overexpression Lysate: 别称
Human AMY2 Overexpression Lysate; Human AMY3 Overexpression Lysate; Human HXA Overexpression Lysate
beta Amylase/AMY2B 背景信息
Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. Alpha-amylase is the major form of amylase found in humans and other mammals. Alpha-amylase hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, yielding glucose and maltose. Amylases is widely expressed and is most prominent in pancreatic juice and saliva, each of which has its own isoform of human α-amylase. They behave differently on isoelectric focusing, and can also be separated in testing by using specific monoclonal antibodies.
amylase, alpha 2B (pancreatic)
Abe A. et al., 2005, FEBS J. 272 (23): 6145-53.
Aghajari N. et al., 1998, Protein Sci. 7 (3): 564-72.
Ramasubbu N. et al., 1996, Acta Crystallographica Section D Biological Crystallography. 52 (3): 435-46.