Human beta Defensin 1/DEFB1 HEK293 Overexpression Lysate

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Human beta Defensin 1/DEFB1 HEK293 Overexpression Lysate: 产品信息

产品描述
This Human beta Defensin 1/DEFB1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of beta Defensin 1/DEFB1 protein (Cat: 15141-H02H) from the overexpression lysate was verified.
表达宿主
HEK293 Cells
种属
Human
蛋白构建信息
A DNA sequence encoding the human DEFB1 (NP_005209.1) (Met1-Lys68) was expressed with the Fc region of human IgG1 at the C-terminus.
分子量
The recombinant human DEFB1 consists 285 amino acids and predicts a molecular mass of 31.8 kDa.

Human beta Defensin 1/DEFB1 HEK293 Overexpression Lysate: 使用指南

制备方法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解缓冲液
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
使用建议
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
缓冲液
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
应用
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human beta Defensin 1/DEFB1 HEK293 Overexpression Lysate: 别称

Human BD1 Overexpression Lysate; Human DEFB-1 Overexpression Lysate; Human DEFB101 Overexpression Lysate; Human HBD1 Overexpression Lysate

beta Defensin 1/DEFB1 背景信息

The DEFB1 gene, encoding for the constitutively expressed human beta-defensin 1 (hBD1) antimicrobial peptide is a potential candidate when studying genetic susceptibility to caries. DEFB1 genetic variations have been reported as contributing to hBD1 production impairment, leading to a greater susceptibility to be infected by oral pathogens, also leading to periodontitis. To counteract host immunity, Cryptosporidium parvum has evolved multiple strategies to suppress host antimicrobial defense. One such strategy is to reduce the production of the antimicrobial peptide beta-defensin 1 (DEFB1) by host epithelial cells. Beta-Defensin-1, an antimicrobial peptide encoded by the DEFB1 gene, is known to play an important role in lung mucosal immunity.
全称
defensin, beta 1
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