Human CD57/B3gat1 HEK293 Overexpression Lysate

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Human CD57/B3gat1 HEK293 Overexpression Lysate: 产品信息

产品描述
This Human CD57/B3gat1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CD57/B3gat1 protein (Cat: 18529-H04H) from the overexpression lysate was verified.
表达宿主
HEK293 Cells
种属
Human
蛋白构建信息
A DNA sequence encoding the human B3GAT1 (NP_473366.1) (Thr28-Ile334) was expressed with the Fc region of mouse IgG1 at the N-terminus.
分子量
The recombinant human B3GAT1 consists of 543 amino acids and predicts a molecular mass of 61.7 kDa.

Human CD57/B3gat1 HEK293 Overexpression Lysate: 使用指南

制备方法
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解缓冲液
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
使用建议
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃.
缓冲液
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
应用
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human CD57/B3gat1 HEK293 Overexpression Lysate: 别称

Human CD57 Overexpression Lysate; Human GLCATP Overexpression Lysate; Human GLCUATP Overexpression Lysate; Human HNK1 Overexpression Lysate; Human LEU7 Overexpression Lysate; Human NK-1 Overexpression Lysate; Human NK1 Overexpression Lysate

CD57/B3gat1 背景信息

B3GAT1 is the key enzyme during the biosynthesis of the carbohydrate epitope HNK-1, which is present on a number of cell adhesion molecules important in neurodevelopment. Saturating concentrations of rVAR2 inhibited downstream integrin signaling, which was mimicked by knockdown of the core chondroitin sulfate synthesis enzymes β-1,3-glucuronyltransferase 1 (B3GAT1) and chondroitin sulfate N-acetylgalactosaminyltransferase 1 (CSGALNACT1).
全称
beta-1,3-glucuronyltransferase 1
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