This Human CD81 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CD81 protein (Cat: 14244-HNCH) from the overexpression lysate was verified.
A DNA sequence encoding the human CD81 (NP_004347.1) (Phe113-Lys201) was expressed with two additional amino acids (Gly&Pro) at the N-terminus.
The recombinant human CD81 comprises 91 amino acids and has a predicted molecular mass of 9.9 kDa. The apparent molecular mass of the monomer is approximately 10 kDa in SDS-PAGE under reducing conditions.
Human CD81 HEK293 Overexpression Lysate: 使用指南
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human CD81 HEK293 Overexpression Lysate: 别称
Human CVID6 Overexpression Lysate; Human S5.7 Overexpression Lysate; Human TAPA1 Overexpression Lysate; Human TSPAN28 Overexpression Lysate
CD81, also known as TAPA-1, belongs to the transmembrane 4 superfamily, also known as the tetraspanin family. Members of this family mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility.CD81 is a widely expressed cell-surface protein involved in an astonishing variety of biologic responses. It is related to adhesion, morphology, activation, proliferation, and differentiation of B, T, and other cells. On B cells CD81 is part of a complex with CD21, CD19, and Leu13. This complex reduces the threshold for B cell activation via the B cell receptor by bridging Ag specific recognition and CD21-mediated complement recognition.
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Bartosch B. et al., 2003, The Journal of Biological Chemistry. 278 (43): 41624-30.
Clark KL. et al., 2001, Journal of Immunology. 167 (9): 5115-21.