Human CDC42BPB Baculovirus-Insect cells Overexpression Lysate: 产品信息
This Human CDC42BPB overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of CDC42BPB protein (Cat: 11540-H20B) from the overexpression lysate was verified.
A DNA sequence encoding the amino acid (Met 1-His 427) of human CDC42BPB (Q9Y5S2) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
The recombinant human CDC42BPB/GST chimera consists of 709 amino acids and has a calculated molecular mass of 82.4 kDa. It migrates as an approximately 70 kDa band in SDS-PAGE under reducing conditions.
Human CDC42BPB Baculovirus-Insect cells Overexpression Lysate: 使用指南
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human CDC42BPB Baculovirus-Insect cells Overexpression Lysate: 别称
Human MRCKB Overexpression Lysate
CDC42BPB is a member of the serine / threonine protein kinase family that contains a Cdc42 / Rsc-binding p21 binding domain similar to that of PAK kinase. The kinase domain of this protein is related to the myotonic dystrophy kinase related ROK and this kinase may have functions in downstream regulating of Cdc42 in cytoskeletal recognization. It has been reported that the CDC42BPB protein take part in regulating numerous cellular functions by binding to members of a serine / threonine protein kinase subfamily. These functions include the remodeling of the cell cytoskeleton that is a feature of cell growth and differentiation.
CDC42 binding protein kinase beta (DMPK-like)
Moncrieff CL, et al. (1999) Cloning and Chromosomal Localization of Human Cdc42-Binding Protein Kinase beta.Genomics. 57 (2): 297-300.
Boyd Y, et al. (2001) Orthologs of seven genes (AKT1, CDC42BPB, DIO3, EIF5, JAG2, KLC, NDUFB1) from human Chromosome 14q32 map to distal chicken Chromosome 5. Mammalian Genome. 13 (2): 120-2.