Human CGB5 HEK293 Overexpression Lysate


Human CGB5 HEK293 Overexpression Lysate: 产品信息

This Human CGB5 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CGB5 protein (Cat: 14574-H05H) from the overexpression lysate was verified.
HEK293 Cells
A DNA sequence encoding the human CGB5 (NP_000728.1) (Met1-Gln165) was expressed with the Fc region of mouse IgG1 at the C-terminus.
The recombinant human CGB5/mFc comprises 379 amino acids and has a predicted molecular mass of 41.9 kDa. The apparent molecular mass of the monomer is approximately 56 kDa in SDS-PAGE under reducing conditions.

Human CGB5 HEK293 Overexpression Lysate: 使用指南

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human CGB5 HEK293 Overexpression Lysate: 别称

Human CGB Overexpression Lysate; Human CGB5 Overexpression Lysate; Human HCG Overexpression Lysate; Human hCGB Overexpression Lysate

CGB5 背景信息

The human CGB5 gene encodes chorionic gonadotropin (hCG)beta 5, which is aberrantly expressed in trophoblastic neoplasm and in some non-trophoblastic neoplasms. Fucntional studies observed that it involved tumor initiation, growth, and metastatic outgrowth. Carriage of particular variants in the promoter of the CGB5 gene seems to protect against RM.
chorionic gonadotropin beta subunit 5
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