This Human CGB5 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CGB5 protein (Cat: 14574-H05H) from the overexpression lysate was verified.
A DNA sequence encoding the human CGB5 (NP_000728.1) (Met1-Gln165) was expressed with the Fc region of mouse IgG1 at the C-terminus.
The recombinant human CGB5/mFc comprises 379 amino acids and has a predicted molecular mass of 41.9 kDa. The apparent molecular mass of the monomer is approximately 56 kDa in SDS-PAGE under reducing conditions.
Human CGB5 HEK293 Overexpression Lysate: 使用指南
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human CGB5 HEK293 Overexpression Lysate: 别称
Human CGB Overexpression Lysate; Human CGB5 Overexpression Lysate; Human HCG Overexpression Lysate; Human hCGB Overexpression Lysate
The human CGB5 gene encodes chorionic gonadotropin (hCG)beta 5, which is aberrantly expressed in trophoblastic neoplasm and in some non-trophoblastic neoplasms. Fucntional studies observed that it involved tumor initiation, growth, and metastatic outgrowth. Carriage of particular variants in the promoter of the CGB5 gene seems to protect against RM.