Human CHN1 Baculovirus-Insect cells Overexpression Lysate: 产品信息
This Human CHN1 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of CHN1 protein (Cat: 14914-HNCB) from the overexpression lysate was verified.
A DNA sequence encoding the human CHN1 (NP_001813.1) (Ala2-Phe459) was fused with two additional amino acids (Gly&Pro) at the N-terminus.
The recombinant human CHN1 consists of 460 amino acids and has a calculated molecular mass of 53.2 kDa. The recombinant protein migrates as an approximately 45 kDa band in SDS-PAGE under reducing conditions.
Human CHN1 Baculovirus-Insect cells Overexpression Lysate: 使用指南
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human CHN1 Baculovirus-Insect cells Overexpression Lysate: 别称
Human ARHGAP2 Overexpression Lysate; Human CHN Overexpression Lysate; Human DURS2 Overexpression Lysate; Human NC Overexpression Lysate; Human RHOGAP2 Overexpression Lysate
CHN1, also known as chimerin 1, is a TPase-activating protein for ras-related p21-rac and a phorbol ester receptor. It is predominantly expressed in neurons, and plays an important role in neuronal signal-transduction mechanisms. CHN1 is involved in the assembly of neuronal locomotor circuits as a direct effector of EPHA4 in axon guidance. The CHN1 gene provides instructions for making two very similar proteins called α1-chimaerin and α2-chimaerin. These proteins play an important role in the early development of the nervous system. In particular, they help regulate complex chemical signaling pathways during the formation and development of nerve cells (neurons). These proteins help guide the growth of axons and dendrites, which are specialized extensions of neurons that transmit and receive nerve impulses throughout the nervous system.
Miyake N. et al, 2010, Am J Med Genet A. 152 (1): 215-7.
Miyake N. et al., 2011, Invest Ophthalmol Vis Sci. 52 (9): 6321-8.