Human Cip4 Baculovirus-Insect cells Overexpression Lysate: 产品信息
This Human Cip4 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of Cip4 protein (Cat: 14476-H08B) from the overexpression lysate was verified.
A DNA sequence encoding the human TRIP10 Isoform 2 (NP_004231.1) (Met1-Asn545) was expressed with a C-terminal polyhistidine tag.
The secreted recombinant human TRIP10 consists of 556 amino acids and predicts a molecular mass of 64 KDa. The apparent molecular mass of the protein is approximately 84.2 KDa in SDS-PAGE under reducing conditions.
Human Cip4 Baculovirus-Insect cells Overexpression Lysate: 使用指南
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.