Human CREB3L1 HEK293 Overexpression Lysate

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Human CREB3L1 HEK293 Overexpression Lysate: 产品信息

产品描述
This Human CREB3L1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CREB3L1 protein (Cat: 12712-H08H) from the overexpression lysate was verified.
表达宿主
HEK293 Cells
种属
Human
蛋白构建信息
A DNA sequence encoding the human CREB3L1 (Q96BA8-1) (Glu396-Ser519) was expressed with a polyhistidine tag at the C-terminus.
分子量
The recombinant human CREB3L1 consists of 135 amino acids and predicts a molecular mass of 15.2 KDa. It migrates as an approximately 21-31 KDa band in SDS-PAGE under reducing conditions.

Human CREB3L1 HEK293 Overexpression Lysate: 使用指南

制备方法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解缓冲液
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
使用建议
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
缓冲液
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
应用
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human CREB3L1 HEK293 Overexpression Lysate: 别称

Human OASIS Overexpression Lysate; Human PSEC0238 Overexpression Lysate

CREB3L1 背景信息

CREB3L1, also known as OASIS, is a cellular transcription factor synthesized as a membrane-bound precursor. It is a putative endoplasmic reticulum (ER) stress sensor in astrocytes with a mechanism of activation. OASIS mRNA expression was detected in pancreatic β-cell lines and rodent islets, and the expression level was up-regulated by ER stress-inducing compounds. CREB3L1 may have a role in pancreas development. CREB3L1 may also play an important role in limiting virus spread by inhibiting proliferation of virus-infected cells. In vitro, CREB3L1 binds to box-B element, cAMP response element (CRE) and CRE-like sequences, and activates transcription through box-B element but not through CRE. It may play a role in gliosis.
全称
cAMP responsive element binding protein 3-like 1
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  • T Cell Receptor Signaling Pathway
    T Cell Receptor Signaling Pathway
参考文献
  • Denard B. et al., 2011, Cell Host Microbe. 10 (1): 65-74.
  • Debelenko LV. et al., 2011, 50 (12): 1054-62.
  • Vellanki. et al., 2010, Endocrinology. 151 (9): 4146.
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