Human DEC-205 HEK293 Overexpression Lysate


Human DEC-205 HEK293 Overexpression Lysate: 产品信息

This Human DEC-205 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of DEC-205 protein (Cat: 16490-H08H) from the overexpression lysate was verified.
HEK293 Cells
A DNA sequence encoding the human LY75 (NP_002340.2) (Ala32-Gly1664) was expressed with a polyhistidine tag at the C-terminus.
The recombinant human LY75 consists 1644 amino acids and predicts a molecular mass of 189.7 kDa.

Human DEC-205 HEK293 Overexpression Lysate: 使用指南

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human DEC-205 HEK293 Overexpression Lysate: 别称

Human CD205 Overexpression Lysate; Human CLEC13B Overexpression Lysate; Human DEC-205 Overexpression Lysate; Human GP200-MR6 Overexpression Lysate; Human LY-75 Overexpression Lysate

DEC-205 背景信息

LY75 (Lymphocyte Antigen 75) is a Protein Coding gene. It is broadly expressed in the lymph node, appendix, and other tissues. LY75 knockdown in SKOV3 cells resulted in predominant upregulation of functional pathways implicated in cell proliferation and metabolism, while pathways associated with cell signaling and adhesion, complement activation, and immune response were mostly suppressed. Moreover, LY75 suppression had an opposite effect on EOC cell lines with the epithelial phenotype (A2780s and OV2008), by directing epithelial-to-mesenchymal transition (EMT) associated with reduced capacity for in vivo EOC cell colonization, as similar/identical signaling pathways were reversely regulated, when compared to mesenchymal LY75 knockdown EOC cells. Diseases associated with LY75 include Pneumonic Plague and Adenoiditis.
lymphocyte antigen 75
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