Human DPYS Baculovirus-Insect cells Overexpression Lysate: 产品信息
This Human DPYS overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of DPYS protein (Cat: 14884-HNCB) from the overexpression lysate was verified.
A DNA sequence encoding the human DPYS (NP_001376.1) (Met1-Pro519) was fused with two additional amino acids (Gly&Pro) at the N-terminus.
The recombinant human DPYS consists of 521 amino acids and has a calculated molecular mass of 56.8 kDa. The recombinant protein migrates as an approximately 54 kDa band in SDS-PAGE under reducing conditions.
Human DPYS Baculovirus-Insect cells Overexpression Lysate: 使用指南
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human DPYS Baculovirus-Insect cells Overexpression Lysate: 别称
Human DHP Overexpression Lysate; Human DHPase Overexpression Lysate
DPYS, also known as dihydropyrimidinase, belongs to the DHOase family, hydantoinase/dihydropyrimidinase subfamily. DPYS catalyzes the second step of the reductive pyrimidine degradation, the reversible hydrolytic ring opening of dihydropyrimidines. It can catalyzes the ring opening of 5,6-dihydrouracil to N-carbamyl-alanine and of 5,6-dihydrothymine to N-carbamyl-amino isobutyrate. DPYS is expressed at a high level in liver and kidney as a major 2.5-kb transcript and a minor 3.8-kb transcript. Defects in the DPYS gene are linked to dihydropyrimidinuria.
Thomas HR. et al., 2008, Genomics. 18 (1): 25-35.
Thomas HR. et al., 2008, Pharmacogenet Genomics. 17 (11): 973-87.
Van Kuilenburg AB. et al., 2007, Mol Genet Metab. 91 (2): 157-64.