Human EGFL6 Baculovirus-insect cells Overexpression Lysate

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Human EGFL6 Baculovirus-insect cells Overexpression Lysate: 产品信息

产品描述
This Human EGFL6 overexpression lysate was created in Baculovirus-insect cells and intented for use as a Western blot (WB) positive control. Purification of EGFL6 protein (Cat: 10867-H18B) from the overexpression lysate was verified.
表达宿主
Baculovirus-insect cells
种属
Human
蛋白构建信息
A DNA sequence encoding the human EGFL6 (NP_056322.2) (Asn22-Asp553) was expressed with the flag tag at the N-terminus and the histidine tag at the C-terminus.
分子量
The recombinant human EGFL6 consists of 551 amino acids and has a calculated molecular mass of 61.5 kDa.

Human EGFL6 Baculovirus-insect cells Overexpression Lysate: 使用指南

制备方法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解缓冲液
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
使用建议
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
缓冲液
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
应用
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human EGFL6 Baculovirus-insect cells Overexpression Lysate: 别称

Human MAEG Overexpression Lysate; Human W80 Overexpression Lysate

EGFL6 背景信息

EGFL6, also known as EGF-L6, belongs to the epidermal growth factor (EGF) repeat superfamily. Members of this superfamily are characterized by the presence of EGF-like repeats and are often involved in the regulation of cell cycle, proliferation, and developmental processes. EGFL6 contains a signal peptide, suggesting that it is secreted; an EGF repeat region consisting of 4 complete EGF-like repeats and 1 partial EGF-like repeat, 3 of which have a calcium-binding consensus sequence; an arg-gly-asp integrin association motif; and a MAM domain, which is believed to have an adhesive function. EGFL6 gene is expressed early during development, and its expression has been detected in lung and meningioma tumors.
全称
EGF-like-domain, multiple 6
参考文献
  • Kimura K. et al., 2006, Genome Res. 16 (1): 55-65.
  • Lee SH. et al., 2008, Pathology. 40 (1): 95-7.
  • Oberauer R. et al., 2010, Mol Cell Biochem. 343 (1-2): 257-69.
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