This Human FAM150A overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of FAM150A protein (Cat: 15606-H05H) from the overexpression lysate was verified.
A DNA sequence encoding the human FAM150A (NP_997296.1) (Met1-Thr129) was expressed with the Fc region of mouse IgG1 at the C-terminus.
The recombinant human FAM150A consists 0 amino acids and predicts a molecular mass of 0 kDa.
Human FAM150A HEK293 Overexpression Lysate: 使用指南
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.