Human FAM3C HEK293 Overexpression Lysate

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Human FAM3C HEK293 Overexpression Lysate: 产品信息

产品描述
This Human FAM3C overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of FAM3C protein (Cat: 15678-H02H) from the overexpression lysate was verified.
表达宿主
HEK293 Cells
种属
Human
蛋白构建信息
A DNA sequence encoding the human FAM3C (Q92520)(Met1-Asp227) was expressed with the Fc region of human IgG1 at the C-terminus.
分子量
The recombinant human FAM3C/Fc is a disulfide-linked homodimer. The reduced monomer comprises 444 amino acids and has a predicted molecular mass of 49.2 kDa. The apparent molecular mass of the protein is approximately 53 and 35 kDa in SDS-PAGE under reducing conditions.

Human FAM3C HEK293 Overexpression Lysate: 使用指南

制备方法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解缓冲液
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
使用建议
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
缓冲液
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
应用
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human FAM3C HEK293 Overexpression Lysate: 别称

Human GS3786 Overexpression Lysate; Human ILEI Overexpression Lysate

FAM3C 背景信息

FAM3C, also known as ILEI, is a member of theFAM3 family. It can be detected in resent in most secretory epithelia. FAM3C may be involved in retinal laminar formation. It promotes epithelial to mesenchymal transition. A change in expression of FAM3C has been noted in pancreatic cancer-derived cells. ILEI is overexpressed and/or altered in intracellular localization in multiple human tumors, an event strongly correlated to invasion/EMT, metastasis formation, and survival in human colon and breast cancer.
全称
family with sequence similarity 3, member C
参考文献
  • Zhu Y. et al., 2002, Genomics. 80 (2): 144-50.
  • Strausberg RL. et al., 2003, Proc Natl Acad Sci. 99 (26): 16899-903.
  • Quéméner-Redon S. et al., 2003, Nature. 424 (6945): 157-64.
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