Human FCMR/FAIM3 HEK293 Overexpression Lysate: 产品信息
This Human FCMR/FAIM3 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of FCMR/FAIM3 protein (Cat: 13556-H02H) from the overexpression lysate was verified.
A DNA sequence encoding the human FAIM3 (NP_005440.1) (Met1-Gly251) was expressed with the Fc region of human IgG1 at the C-terminus.
The recombinant human FAIM3/Fc is a disulfide-linked homodimer. The reduced monomer comprises 475 amino acids and has a predicted molecular mass of 53 kDa. The apparent molecular mass of the protein is approximately 66 kDa in SDS-PAGE under reducing conditions.
Human FCMR/FAIM3 HEK293 Overexpression Lysate: 使用指南
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.