Human Frizzled 6 HEK293 Overexpression Lysate

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Human Frizzled 6 HEK293 Overexpression Lysate: 产品信息

产品描述
This Human Frizzled 6 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Frizzled 6 protein (Cat: 16004-H05H) from the overexpression lysate was verified.
表达宿主
HEK293 Cells
种属
Human
蛋白构建信息
A DNA sequence encoding the human FZD6 (NP_003497.2) (Met1-Val153) was expressed with the Fc region of mouse IgG1 at the C-terminus.
分子量
The recombinant human FZD6 consists 369 amino acids and predicts a molecular mass of 42 kDa.

Human Frizzled 6 HEK293 Overexpression Lysate: 使用指南

制备方法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解缓冲液
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
使用建议
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
缓冲液
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
应用
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human Frizzled 6 HEK293 Overexpression Lysate: 别称

Human FZ-6 Overexpression Lysate; Human FZ6 Overexpression Lysate; Human HFZ6 Overexpression Lysate; Human NDNC10 Overexpression Lysate

Frizzled 6 背景信息

FZD6 plays a pivotal role in the growth and guidance of the nail plate in humans by acting as a molecular switch between different Wnt pathways. Inherited isolated nail anomaly manifesting with onychauxis and onycholysis is a rare condition, caused by mutations in the gene FZD6, encoding membrane-bound Wnt receptor protein. The overexpression of FZD6 may play an important role in the development of Colorectal cancer (CRC). FZD6 repressed gastric cancer cell proliferation and migration via activating non-canonical wnt pathway. FZD6 plays crucial roles in human tumorigenesis.
全称
frizzled class receptor 6
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