Human GALNT10 HEK293 Overexpression Lysate


Human GALNT10 HEK293 Overexpression Lysate: 产品信息

This Human GALNT10 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of GALNT10 protein (Cat: 16046-H07H) from the overexpression lysate was verified.
HEK293 Cells
A DNA sequence encoding the human GALNT10 (NP_938080.1) (Thr39-Asn603) was expressed with an N-terminal polyhistidine tag.
The recombinant human GALNT10 comprises 584 amino acids and has a predicted molecular mass of 67 kDa. The apparent molecular mass of the protein is approximately 63.5 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Human GALNT10 HEK293 Overexpression Lysate: 使用指南

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human GALNT10 HEK293 Overexpression Lysate: 别称

Human GALNACT10 Overexpression Lysate; Human PPGALNACT10 Overexpression Lysate; Human PPGANTASE10 Overexpression Lysate

GALNT10 背景信息

Ectopic expression and knockdown studies showed that GALNT10 indeed promotes proliferation and apoptosis resistance of hepatoma cells in a glycosyltransferase-dependent manner. The genetic variants on LEKR1 and GALNT10 genes have been associated with control of adiposity and weight.
polypeptide N-acetylgalactosaminyltransferase 10
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