Human Gastrokine 1 Baculovirus-Insect cells Overexpression Lysate


Human Gastrokine 1 Baculovirus-Insect cells Overexpression Lysate: 产品信息

This Human Gastrokine 1 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of Gastrokine 1 protein (Cat: 16115-H08B) from the overexpression lysate was verified.
Baculovirus-Insect cells
A DNA sequence encoding the human GKN1 (Q9NS71) (Asn35-Asn199) was expressed with a C-terminal polyhistidine tag.
The secreted recombinant human GKN1 consists of 175 amino acids and predicts a molecular mass of 19.6 KDa. The apparent molecular mass of the protein is approximately 24 KDa in SDS-PAGE under reducing conditions.

Human Gastrokine 1 Baculovirus-Insect cells Overexpression Lysate: 使用指南

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human Gastrokine 1 Baculovirus-Insect cells Overexpression Lysate: 别称

Human AMP18 Overexpression Lysate; Human BRICD1 Overexpression Lysate; Human CA11 Overexpression Lysate; Human FOV Overexpression Lysate; Human foveolin Overexpression Lysate; Human UNQ489/PRO1005 Overexpression Lysate

Gastrokine 1 背景信息

Gastrokine 1 (GKN1) belongs to the BRICHOS domain family and plays a major role in maintaining gastric mucosa integrity. GKN1 is highly expressed in gastric tissue and is secreted into the stomach but is not expressed in gastric cancer. GKN1-mediated inhibition of APP processing might represent a new approach for the prevention and therapy of Alzheimer's disease (AD). In the presence of GKN2, GKN1 loses its ability to decrease cell proliferation, induce apoptosis, and inhibit epigenetic alterations in gastric cancer cells, that GKN2 may contribute to the homeostasis of gastric epithelial cells by inhibiting GKN1 activity. The loss of GKN1 function contributes to malignant transformation and proliferation of gastric epithelial cells in gastric carcinogenesis.
gastrokine 1
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