Human GNRH2 HEK293 Overexpression Lysate


Human GNRH2 HEK293 Overexpression Lysate: 产品信息

This Human GNRH2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of GNRH2 protein (Cat: 15432-H01H) from the overexpression lysate was verified.
HEK293 Cells
A DNA sequence encoding the human GNRH2 (NP_001492.1) (Gln24-Val120) was expressed with the Fc region of human IgG1 at the N-terminus.
The recombinant human GNRH2 consists 357 amino acids and predicts a molecular mass of 39 kDa.

Human GNRH2 HEK293 Overexpression Lysate: 使用指南

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human GNRH2 HEK293 Overexpression Lysate: 别称

Human GnRH-II Overexpression Lysate; Human GNRH2 Overexpression Lysate; Human LH-RHII Overexpression Lysate

GNRH2 背景信息

GNRH2 (Gonadotropin-Releasing Hormone 2) is a Protein Coding gene. This gene is a member of the gonadotropin-releasing hormone (GnRH) gene family. Proteins encoded by members of this gene family are proteolytically cleaved to form neuropeptides which, in part, regulate reproductive functions by stimulating the production and release of the gonadotropins follicle-stimulating hormone (FSH) and luteinizing hormone (LH). 3 alternatively spliced human isoforms have been reported. The second mammalian isoform (GNRH2) is an ineffective stimulant of gonadotropin release. Given that pigs lack testicular GNRHR1, these data may indicate that GNRH2 and its receptor are involved in the autocrine or paracrine regulation of testosterone secretion. Diseases associated with GNRH2 include Colon Mucinous Adenocarcinoma and Ovarian Cancer.
gonadotropin releasing hormone 2
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