Human GPIHBP1 HEK293 Overexpression Lysate


Human GPIHBP1 HEK293 Overexpression Lysate: 产品信息

This Human GPIHBP1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of GPIHBP1 protein (Cat: 15388-H02H) from the overexpression lysate was verified.
HEK293 Cells
A DNA sequence encoding the human GPIHBP1 (AAH63857.1) (Met1-Ser160) was expressed with the Fc region of human IgG1 at the C-terminus.
The recombinant human GPIHBP1 consists 378 amino acids and predicts a molecular mass of 42.2 kDa.

Human GPIHBP1 HEK293 Overexpression Lysate: 使用指南

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human GPIHBP1 HEK293 Overexpression Lysate: 别称

Human GPI-HBP1 Overexpression Lysate; Human HYPL1D Overexpression Lysate

GPIHBP1 背景信息

GPIHBP1 (Glycosylphosphatidylinositol Anchored High-Density Lipoprotein Binding Protein 1) is a Protein Coding gene. The encoded protein is a member of the lymphocyte antigen 6 (Ly6) family. GPIHBP1 is an endothelial cell protein, which binds LPL in the subendothelial spaces and transports it to the capillary lumen. GPIHBP1 was positively correlated with LPL, and GPIHBP1 is a better marker for bodyweight decrease than LPL. GPIHBP1, a glycosylphosphatidylinositol (GPI)-anchored protein of capillary endothelial cells, transports lipoprotein lipase to the capillary lumen and is essential for the lipolytic processing of triglyceride-rich lipoproteins. Diseases associated with GPIHBP1 include Hyperlipoproteinemia, Type Id, and Hyperlipoproteinemia, Type I.
glycosylphosphatidylinositol anchored high density lipoprotein binding protein 1
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