This Human GPR56 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of GPR56 protein (Cat: 11017-H08H) from the overexpression lysate was verified.
A DNA sequence encoding the human GPR56 isoform b (NP_958933.1) extracellular domain (Met 1-Val 342) was fused with a polyhistidine tag at the C-terminus.
The secreted recombinant human GPR56 comprises 328 amino acids and has a predicted molecular mass of 37.4 kDa. As a result of glycosylation, the apparent molecular mass of rhGPR56 is approximately 50-60 kDa in SDS-PAGE under reducing conditions.
Human GPR56 HEK293 Overexpression Lysate: 使用指南
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human GPR56 HEK293 Overexpression Lysate: 别称
Human BFPP Overexpression Lysate; Human DKFZp781L1398 Overexpression Lysate; Human TM7LN4 Overexpression Lysate; Human TM7XN1 Overexpression Lysate; Human UNQ540/PRO1083 Overexpression Lysate
ADGRG1 (Adhesion G Protein-Coupled Receptor G1, also known as GPR56) is a Protein Coding gene. GPR56 is a member of an adhesion G protein-coupled receptor family with a very long N-terminal stalk and seven transmembrane domains. The encoded protein binds specifically to transglutaminase 2, a component of tissue and tumor stroma implicated as an inhibitor of tumor progression. GPR56 may be a target for the treatment of type 2 diabetes. GPR56 inhibits melanoma metastatic growth by impeding the expansion of micrometastases to macrometastases. GPR56 loss of function promotes mesenchymal differentiation and radioresistance of glioma initiating cells both in vitro and in vivo. Diseases associated with ADGRG1 include Polymicrogyria, Bilateral Frontoparietal, and Polymicrogyria, Bilateral Perisylvian, Autosomal Recessive.
adhesion G protein-coupled receptor G1
Liu M., et al., 1999, Genomics 55: 296-305.
Huang,Y. et al., 2008, Mol Cell Biochem. 308 (1-2):133-9.