Human GPX7 HEK293 Overexpression Lysate

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Human GPX7 HEK293 Overexpression Lysate: 产品信息

产品描述
This Human GPX7 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of GPX7 protein (Cat: 12651-H02H) from the overexpression lysate was verified.
表达宿主
HEK293 Cells
种属
Human
蛋白构建信息
A DNA sequence encoding the human GPX7 (NP_056511.2) (Met1-Leu187) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
分子量
The recombinant human GPX7/Fc is a disulfide-linked homodimer. The reduced monomer comprises 408 amino acids and has a predicted molecular mass of 46.1 kDa. The apparent molecular mass of the protein is approximately kDa in SDS-PAGE under reducing conditions.

Human GPX7 HEK293 Overexpression Lysate: 使用指南

制备方法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解缓冲液
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
使用建议
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
缓冲液
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
应用
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human GPX7 HEK293 Overexpression Lysate: 别称

Human CL683 Overexpression Lysate; Human GPx-7 Overexpression Lysate; Human GPX6 Overexpression Lysate; Human GSHPx-7 Overexpression Lysate; Human NPGPx Overexpression Lysate; Human UNQ469/PRO828 Overexpression Lysate

GPX7 背景信息

GPX7 gene contains 3 distinct gt-ag introns. Transcription produces 4 different mRNAs, 3 alternatively spliced variants, and 1 unspliced form. There are 5 validated alternative polyadenylation sites. The mRNAs appear to differ by overlapping exons with different boundaries. GPX7 is an enzyme. It has molecular functions (glutathione peroxidase activity, oxidoreductase activity) and to localize in various compartments (extracellular space, extracellular region). GPX7 gene has been proposed to participate in pathways (Arachidonic acid metabolism, Glutathione metabolism), and processes (oxidation-reduction, response to oxidative stress). GPX7 modulates the bone turnover after ovariectomy in rats, it does not compensate for the action of estrogen after ovariectomy in rats. It has been shown that three mAbs (GPX7, GPX22, and GPZ35) inhibit IL-6-mediated biological responses such as Ig production in a human B cell line and proliferative responses of a human Lennert's lymphoma-derived T cell line, a human myeloma cell line, and a mouse pro-B cell line-derived transfectant expressing human gp130.
全称
glutathione peroxidase 7
参考文献
  • Rual JF, et al. (2005) Towards a proteome-scale map of the human protein-protein interaction network. Nature. 437(7062):1173-8.
  • Gregory SG, et al. (2006) The DNA sequence and biological annotation of human chromosome 1. Nature. 441(7091):315-21.
  • Lim J, et al. (2006) A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration. Cell. 125(4):801-14.
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