Human Her2/ERBB2 HEK293 Overexpression Lysate


Human Her2/ERBB2 HEK293 Overexpression Lysate: 产品信息

This Human Her2/ERBB2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Her2/ERBB2 protein (Cat: 10004-H08H1) from the overexpression lysate was verified.
HEK293 Cells
A DNA sequence encoding the human ERBB2 (AAA75493.1) (Met1-Cys195) was expressed with a polyhistidine tag at the C-terminus.
The recombinant human ERBB2 consists of 184 amino acids and predicts a molecular mass of 20.9 kDa.

Human Her2/ERBB2 HEK293 Overexpression Lysate: 使用指南

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human Her2/ERBB2 HEK293 Overexpression Lysate: 别称

Human CD340 Overexpression Lysate; Human ENV Overexpression Lysate; Human ENVW Overexpression Lysate; Human ERVWE1 Overexpression Lysate; Human HER-2 Overexpression Lysate; Human HER-2/neu Overexpression Lysate; Human HER2 Overexpression Lysate; Human HERV-7q Overexpression Lysate; Human HERV-W-ENV Overexpression Lysate; Human HERV7Q Overexpression Lysate; Human HERVW Overexpression Lysate; Human HERVWENV Overexpression Lysate; Human MLN 19 Overexpression Lysate; Human MLN19 Overexpression Lysate; Human NEU Overexpression Lysate; Human NGL Overexpression Lysate; Human TKR1 Overexpression Lysate

Her2/ERBB2 背景信息

Epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD34, is a type I membrane glycoprotein, and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.
erb-b2 receptor tyrosine kinase 2
  • Krawczyk N, et al. (2009) HER2 status on persistent disseminated tumor cells after adjuvant therapy may differ from initial HER2 status on primary tumor. Anticancer Res. 29(10): 4019-24.
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