Human HNT/NTM HEK293 Overexpression Lysate


Human HNT/NTM HEK293 Overexpression Lysate: 产品信息

This Human HNT/NTM overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of HNT/NTM protein (Cat: 15169-H08H) from the overexpression lysate was verified.
HEK293 Cells
A DNA sequence encoding the human NTM (NP_001137531.1) (Met1-Gly312) was expressed with a polyhistidine tag at the C-terminus.
The recombinant human NTM consists 290 amino acids and predicts a molecular mass of 32.3 kDa.

Human HNT/NTM HEK293 Overexpression Lysate: 使用指南

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human HNT/NTM HEK293 Overexpression Lysate: 别称

Human HNT Overexpression Lysate; Human IGLON2 Overexpression Lysate; Human NTRI Overexpression Lysate

HNT/NTM 背景信息

NTM (Neurotrimin) is a Protein Coding gene. 4 alternatively spliced human isoforms have been reported. This gene encodes a member of the IgLON (LAMP, OBCAM, Ntm) family of immunoglobulin (Ig) domain-containing glycosylphosphatidylinositol (GPI)-anchored cell adhesion molecules. IgLONs have been associated with psychiatric disorders, intelligence, body weight, heart disease, and tumors. The encoded protein may promote neurite outgrowth and adhesion via a homophilic mechanism. Neurotrimin is a member of the family of neural cell adhesion molecules. Its expression pattern suggests that Ntm promotes axonal fasciculation, guides nerve fibers to specific targets and stabilizes synapses as it accumulates coincident with synaptogenesis. Diseases associated with NTM include Connective Tissue Disease and Jacobsen Syndrome.
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