Human IL-1 alpha/IL1A HEK293 Overexpression Lysate: 产品信息
This Human IL-1 alpha/IL1A overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of IL-1 alpha/IL1A protein (Cat: 10128-HNCH) from the overexpression lysate was verified.
The processed form of human IL1α (NP_000566.3) (Ser 113-Ala 271) was expressed and purified.
The recombinant human IL1α consists of 159 amino acids and has a predicted molecular mass of 18 kDa. As a result of glycosylation, the apparent molecular mass of rhIL1α is approximately 22 kDa in SDS-PAGE under reducing conditions.
Human IL-1 alpha/IL1A HEK293 Overexpression Lysate: 使用指南
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human IL-1 alpha/IL1A HEK293 Overexpression Lysate: 别称
Human IL-1A Overexpression Lysate; Human IL-1F1 Overexpression Lysate; Human IL1 Overexpression Lysate; Human IL1-ALPHA Overexpression Lysate; Human IL1F1 Overexpression Lysate
IL-1 alpha/IL1A 背景信息
IL-1 alpha is a member of the interleukin 1 cytokine family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. Cytokines can be classified into two groups: pro- and anti-inflammatory. Pro-inflammatory cytokines, including IFNgamma, IL-1, IL-6 and TNF-alpha, are predominantly derived from the innate immune cells and Th1 cells. Anti-inflammatory cytokines, including IL-1, IL-4, IL-13 and IL-5, are synthesized from Th2 immune cells. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. It is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. IL-1 alpha stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity.