This Human IL10RB overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of IL10RB protein (Cat: 10945-H08H) from the overexpression lysate was verified.
A DNA sequence encoding the extracellular domainof human IL10Rβ (NP_000619.3) (Met 1-Ser 220) was expressed, fused with a polyhistidine tag at the C-terminus.
The recombinant human IL10Rβ comprises 212 amino acids and has a predicted molecular mass of 25 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhIL10Rβ is approximately 45-48 kDa due to glycosylation.
Human IL10RB HEK293 Overexpression Lysate: 使用指南
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human IL10RB HEK293 Overexpression Lysate: 别称
Human CDW210B Overexpression Lysate; Human CRF2-4 Overexpression Lysate; Human CRFB4 Overexpression Lysate; Human D21S58 Overexpression Lysate; Human D21S66 Overexpression Lysate; Human IL-10R2 Overexpression Lysate
Interleukin 10 receptor, beta subunit (IL10RB/IL-10RB) also known as Cytokine receptor family 2 member 4, Interleukin-10 receptor subunit 2, and cytokine receptor family II, member 4, is a subunit for the interleukin-10 receptor. IL10RB/IL-10RB belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex. Coexpression of this and IL10RA proteins is required for IL10-induced signal transduction. Defects in IL10RB/IL-10RB are the cause of inflammatory bowel disease type 25 (IBD25). It is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn's disease and ulcerative colitis phenotypes. Crohn's disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.