This Human IL1RL2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of IL1RL2 protein (Cat: 14398-H08H) from the overexpression lysate was verified.
A DNA sequence encoding the human IL1RL2 (NP_003845.2) (Met1-Tyr337) was expressed with a polyhistidine tag at the C-terminus.
The recombinant human IL1RL2 consists 329 amino acids and predicts a molecular mass of 37.6 kDa.
Human IL1RL2 HEK293 Overexpression Lysate: 使用指南
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human IL1RL2 HEK293 Overexpression Lysate: 别称
Human IL-1Rrp2 Overexpression Lysate; Human IL-36R Overexpression Lysate; Human IL1R-rp2 Overexpression Lysate; Human IL1RRP2 Overexpression Lysate
IL1RL2 (Interleukin 1 Receptor-Like 2, also known as IL-36R) is a Protein Coding gene. The protein encoded by this gene is a member of the interleukin 1 receptor family. This gene and four other interleukin 1 receptor family genes form a cytokine receptor gene cluster in a region mapped to chromosome 2q12. The IL36 receptor is an important mediator molecule in the inflammatory response. Animal data suggest that IL1RL2 is involved in mucosal healing. Therapeutic intervention of IL-36R signaling offers an innovative treatment paradigm for targeting epithelial cell-mediated inflammatory diseases such as the life-threatening psoriasis variant called generalized pustular psoriasis (GPP). Diseases associated with IL1RL2 include Familial Cold Autoinflammatory Syndrome 2 and Geographic Tongue.