Human ILT4 HEK293 Overexpression Lysate

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Human ILT4 HEK293 Overexpression Lysate: 产品信息

产品描述
This Human ILT4 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of ILT4 protein (Cat: 14132-H08H) from the overexpression lysate was verified.
表达宿主
HEK293 Cells
种属
Human
蛋白构建信息
A DNA sequence encoding the human LILRB2 (AAH36827.1) (Met1-Val461) was expressed with a polyhistidine tag at the C-terminus.
分子量
The recombinant human LILRB2 consists of 451 amino acids and predicts a molecular mass of 49.2 KDa. It migrates as an approximately 66 KDa band in SDS-PAGE under reducing conditions.

Human ILT4 HEK293 Overexpression Lysate: 使用指南

制备方法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解缓冲液
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
使用建议
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
缓冲液
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
应用
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human ILT4 HEK293 Overexpression Lysate: 别称

Human CD85D Overexpression Lysate; Human ILT-4 Overexpression Lysate; Human ILT4 Overexpression Lysate; Human LIR-2 Overexpression Lysate; Human LIR2 Overexpression Lysate; Human MIR-10 Overexpression Lysate; Human MIR10 Overexpression Lysate

ILT4 背景信息

ILT4, also known as LILRB2, is a member of the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). ILT4 gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family. Multiple transcript variants encoding different isoforms have been found for the ILT4 gene. ILT4 is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity.
全称
leukocyte immunoglobulin like receptor B2
参考文献
  • Colonna M., et al.,(1997), A common inhibitory receptor for major histocompatibility complex class I molecules on human lymphoid and myelomonocytic cells. J. Exp. Med. 186:1809-1818.
  • Borges L., et al., (1997), A family of human lymphoid and myeloid Ig-like receptors, some of which bind to MHC class I molecules.J. Immunol. 159:5192-5196.
  • Grimwood J., et al.,(2004), The DNA sequence and biology of human chromosome 19.Nature 428:529-535.
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