Human IP6K1/IHPK1 Baculovirus-Insect cells Overexpression Lysate

Price:
Size:
Number:

Human IP6K1/IHPK1 Baculovirus-Insect cells Overexpression Lysate: 产品信息

产品描述
This Human IP6K1/IHPK1 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of IP6K1/IHPK1 protein (Cat: 13927-H20B) from the overexpression lysate was verified.
表达宿主
Baculovirus-Insect cells
种属
Human
蛋白构建信息
A DNA sequence encoding the human IHPK1 (Q92551-1) (Met1-Gln441) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
分子量
The recombinant human IHPK1 /GST chimera consists of 678 amino acids and has a calculated molecular mass of 78 kDa. The recombinant protein migrates approximately 88 kDa band in SDS-PAGE under reducing conditions.

Human IP6K1/IHPK1 Baculovirus-Insect cells Overexpression Lysate: 使用指南

制备方法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解缓冲液
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
使用建议
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
缓冲液
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
应用
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human IP6K1/IHPK1 Baculovirus-Insect cells Overexpression Lysate: 别称

Human IHPK1 Overexpression Lysate; Human PiUS Overexpression Lysate

IP6K1/IHPK1 背景信息

IP6K1 (Inositol Hexakisphosphate Kinase 1) is a Protein Coding gene. 2 alternatively spliced human isoforms have been reported. IHPK1 is an inositol hexaphosphate kinase (IHPK) protein that belongs to the inositol phosphokinase (IPK) family. IHPK proteins are likely responsible for the conversion of inositol hexakisphosphate (InsP6) to diphosphoinositol pentakisphosphate (InsP7/PP-InsP5). IHPK1 may also convert 1,3,4,5,6-pentakisphosphate (InsP5) to PP-InsP4 and affect the growth-suppressive and apoptotic activities of interferon-beta in some ovarian cancers. During cell death, IHPK1 activity is enhanced, and the intracellular InsP7 level is augmented. The distribution of IHPK1 or another predisposing gene affected by the position effect of translocation may explain the T2DM phenotype at least in this family.
全称
inositol hexakisphosphate kinase 1
参考文献
  • Strausberg RL, et al. (2003) Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci. 99(26):16899-903.
  • Saiardi A, et al. (2001) Identification and characterization of a novel inositol hexakisphosphate kinase. J Biol Chem. 276(42):39179-85.
  • Kamimura J, et al. (2004) The IHPK1 gene is disrupted at the 3p21.31 breakpoint of t(3;9) in a family with type 2 diabetes mellitus. J Hum Genet. 49(7):360-5.
添加购物车成功! 添加购物车失败!请再次尝试 正在更新购物车,请稍后 U.S.A.