This Human ITIH3 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of ITIH3 protein (Cat: 16138-H02H) from the overexpression lysate was verified.
A DNA sequence encoding the human (NP_002208.3) (Met1-Asp651) was expressed with the Fc region of human IgG1 at the C-terminus.
The recombinant human consists 869 amino acids and predicts a molecular mass of 97.7 kDa.
Human ITIH3 HEK293 Overexpression Lysate: 使用指南
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human ITIH3 HEK293 Overexpression Lysate: 别称
Human H3P Overexpression Lysate; Human ITI-HC3 Overexpression Lysate; Human SHAP Overexpression Lysate
ITIH3 (Inter-Alpha-Trypsin Inhibitor Heavy Chain 3) is a Protein Coding gene. This gene encodes the heavy chain subunit of the pre-alpha-trypsin inhibitor complex. This complex may stabilize the extracellular matrix through its ability to bind hyaluronic acid. ITIH3 is involved in the stabilization of the extracellular matrix. A polymorphism (rs2535629) of ITIH3 showed the strongest association signal with susceptibility to psychiatric disorders in Caucasian populations. Common single nucleotide polymorphisms (SNPs) in or near the inter-alpha-trypsin inhibitor heavy chain H3 (ITIH3) gene have been repeatedly associated with schizophrenia and related psychiatric disorders in genome-wide association studies.ITIH3 may be a useful biomarker for the early detection of gastric cancer. Diseases associated with ITIH3 include Schizophrenia and Major Depressive Disorder.