Human KIR2DL1 HEK293 Overexpression Lysate

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Human KIR2DL1 HEK293 Overexpression Lysate: 产品信息

产品描述
This Human KIR2DL1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of KIR2DL1 protein (Cat: 13145-H02H) from the overexpression lysate was verified.
表达宿主
HEK293 Cells
种属
Human
蛋白构建信息
A DNA sequence encoding the human KIR2DL1 (NP_055033.2) extracellular domain (Met 1-His 245) was fused with the Fc region of human IgG1 at the C-terminus.
分子量
The secreted recombinant human KIR2DL1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 465 amino acids and has a predicted molecular mass of 51.7 kDa. The apparent molecular mass of rhKIR2DL1/Fc monomer is approximately 70 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Human KIR2DL1 HEK293 Overexpression Lysate: 使用指南

制备方法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解缓冲液
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
使用建议
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
缓冲液
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
应用
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human KIR2DL1 HEK293 Overexpression Lysate: 别称

Human CD158A Overexpression Lysate; Human KIR-K64 Overexpression Lysate; Human KIR221 Overexpression Lysate; Human NKAT Overexpression Lysate; Human NKAT-1 Overexpression Lysate; Human NKAT1 Overexpression Lysate; Human p58.1 Overexpression Lysate; Human XXbac-BCX195L8.1 Overexpression Lysate; Human XXbac-BPG184J6.7 Overexpression Lysate

KIR2DL1 背景信息

Killer cell immunoglobulin-like receptor 2DL1 or KIR2DL1 is an inhibitory Natural Killer cell immunoglobulin-like receptor with two extracellular immunoglobulin domains. KIR2DL1 is a member of the Killer cell immunoglobulin-like receptor family whose members are classified by the number of the extracellular immunoglobulin domains and the length of the cytoplasm domain. KIR2DL1 is a transmembrane glycoprotein expressed by natural killer cells and subsets of T cells. KIR2DL1 down-regulates the cytotoxicity of NK cells upon recognition of specific class I major histocompatibility complex (MHC) molecules on target cells. It has been reported that the KIR2DL1 is bound to its class I MHC ligand, HLA-Cw4. The KIR2DL1-HLA-Cw4 interface exhibits charge and shape complementarity. Specificity is mediated by a pocket in KIR2DL1 that hosts the Lys80 residue of HLA-Cw4. Many residues conserved in HLA-C and KIR2DL receptors make different interactions in KIR2DL1-HLA-Cw4 and a previously reported KIR2DL2-HLA-Cw3 complex. A dimeric aggregate of KIR-HLA-C complexes was observed in one KIR2DL1-HLA-Cw4 crystal.
全称
killer cell immunoglobulin like receptor, two Ig domains and long cytoplasmic tail 1
参考文献
  • Fan QR, et al. (2001) Crystal structure of the human natural killer cell inhibitory receptor KIR2DL1-HLA-Cw4 complex. Nature Immunology. 2: 452-60.
  • Wagtmann N, et al. (1995) Molecular clones of the p58 NK cell receptor reveal immunoglobulin-related molecules with diversity in both the extra- and intracellular domains. Immunity. 2 (5): 439-49.
  • Colonna M, et al. (1995) Cloning of immunoglobulin-superfamily members associated with HLA-C and HLA-B recognition by human natural killer cells. Science. 268 (5209): 405-8.
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