Human KIR2DL3 HEK293 Overexpression Lysate

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Human KIR2DL3 HEK293 Overexpression Lysate: 产品信息

产品描述
This Human KIR2DL3 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of KIR2DL3 protein (Cat: 12828-H08H) from the overexpression lysate was verified.
表达宿主
HEK293 Cells
种属
Human
蛋白构建信息
A DNA sequence encoding the human KIR2DL3 (AAX23102.1) extracellular domain (Met 1-His 245) was fused with a polyhistidine tag at the C-terminus.
分子量
The secreted recombinant human KIR2DL3 consists of 235 amino acids and has a predicted molecular mass of 25.9 kDa. As a result of glycosylation, the apparent molecular mass of rh KIR2DL3 is approximately 45 kDa in SDS-PAGE under reducing conditions.

Human KIR2DL3 HEK293 Overexpression Lysate: 使用指南

制备方法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解缓冲液
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
使用建议
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
缓冲液
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
应用
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human KIR2DL3 HEK293 Overexpression Lysate: 别称

Human CD158b Overexpression Lysate; Human CD158B2 Overexpression Lysate; Human CU464054.1 Overexpression Lysate; Human GL183 Overexpression Lysate; Human KIR-023GB Overexpression Lysate; Human KIR-K7b Overexpression Lysate; Human KIR-K7c Overexpression Lysate; Human KIR2DS5 Overexpression Lysate; Human KIRCL23 Overexpression Lysate; Human MGC129943 Overexpression Lysate; Human NKAT Overexpression Lysate; Human NKAT2 Overexpression Lysate; Human NKAT2A Overexpression Lysate; Human NKAT2B Overexpression Lysate; Human p58 Overexpression Lysate

KIR2DL3 背景信息

Killer cell immunoglobulin-like receptor 2DL3, also known as CD158 antigen-like family member B2, KIR-23GB, Killer inhibitory receptor cl 2-3, MHC class I NK cell receptor, NKAT2a, NKAT2b, Natural killer-associated transcript 2, p58 natural killer cell receptor clone CL-6, p58.2 MHC class-I-specific NK receptor, CD158b2, and KIR2DL3, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily. KIR2DL3 contains 2 Ig-like C2-type (immunoglobulin-like) domains. KIR2DL3 interacts with ARRB2. KIR2DL3 is a receptor on natural killer (NK) cells for HLA-C alleles (HLA-Cw1, HLA-Cw3, and HLA-Cw7). KIR2DL3 inhibits the activity of NK cells thus preventing cell lysis.
全称
killer cell immunoglobulin like receptor, two Ig domains and long cytoplasmic tail 3
参考文献
  • Selvakumar A., et al., 1997, Immunol. Rev. 155:183-196.
  • Wilson M.J., et al., 1997, Tissue Antigens 49:574-579.
  • Maenaka K., et al., 1999, Structure 7:391-398.
  • Vitale,M. et al., 2004, Int Immunol. 16 (10):1459-66.
  • Yu M.-C., et al., 2008, Nat. Immunol. 9:898-907.
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