Human KIR2DL4 CHO Stable Overexpression Lysate

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Human KIR2DL4 CHO Stable Overexpression Lysate: 产品信息

产品描述
This Human KIR2DL4 overexpression lysate was created in CHO Stable Cells and intented for use as a Western blot (WB) positive control. Purification of KIR2DL4 protein (Cat: 13052-H02S) from the overexpression lysate was verified.
表达宿主
CHO Stable Cells
种属
Human
蛋白构建信息
A DNA sequence encoding the human KIR2DL4 (ADY38409.1)(Met1-His242) was expressed with the Fc region of human IgG1 at the C-terminus.
分子量
The recombinant human KIR2DL4/Fc is a disulfide-linked homodimer. The reduced monomer comprises 462 amino acids and has a predicted molecular mass of 51.3 kDa. The apparent molecular mass of the protein is approximately 62, 34 and 32 kDa in SDS-PAGE under reducing conditions.

Human KIR2DL4 CHO Stable Overexpression Lysate: 使用指南

制备方法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解缓冲液
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
使用建议
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
缓冲液
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
应用
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human KIR2DL4 CHO Stable Overexpression Lysate: 别称

Human CD158D Overexpression Lysate; Human G9P Overexpression Lysate; Human KIR Overexpression Lysate; Human KIR-103AS Overexpression Lysate; Human KIR-2DL4 Overexpression Lysate; Human KIR103 Overexpression Lysate; Human KIR103AS Overexpression Lysate; Human KIR2DL4 Overexpression Lysate

KIR2DL4 背景信息

KIR2DL4, also known as CD158d, is a member of the killer cell Ig-like receptor (KIR) family. KIRs are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous. KIR2DL4 is expressed in all NK cells and some T cells. KIR2DL4 activates the cytotoxicity of NK cells, despite the presence of an immunoreceptor tyrosine-based inhibition motif (ITIM) in its cytoplasmic tail. The ITIM was not necessary for activation of lysis by KIR2DL4. The activation signal of KIR2DL4 was sensitive to inhibition by another ITIM-containing receptor. The activation-deficient mutant of KIR2DL4 inhibited the signal delivered by the activating receptor CD16.
全称
killer cell immunoglobulin like receptor, two Ig domains and long cytoplasmic tail 4
参考文献
  • Selvakumar A. et al., 1997, Tissue Antigens. 48 (4 Pt 1): 285-94.
  • Selvakumar A. et al., 1997, Immunol Rev. 155: 183-96.
  • Selvakumar A. et al., 1997, Tissue Antigens. 49 (6): 564-73.
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